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grnas n a recombinant dna lenti dcas9 vp64 addgene  (Addgene inc)


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    Addgene inc grnas n a recombinant dna lenti dcas9 vp64 addgene
    Grnas N A Recombinant Dna Lenti Dcas9 Vp64 Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 358 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/addgene+dcas9+vp64/lenti+dCAS-VP64_Blast+(Plasmid+%2361425)/pm41856116-659-103-108
    Average 96 stars, based on 358 article reviews
    grnas n a recombinant dna lenti dcas9 vp64 addgene - by Bioz Stars, 2026-11
    96/100 stars

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    Expressing:

    Article Title: Specific modulation of CRISPR transcriptional activators through RNA-sensing guide RNAs in mammalian cells and zebrafish embryos
    Article Snippet: .. All mammalian plasmids expressing Cas9 and deadCas9-fused transcriptional activators were acquired from Addgene: dCas9-Vp64 (#47107), dCas9-VPR (#63798) as well as Cas9_pX458 (#48138). .. Native sgRNAs, iSBH-sgRNAs, RNA triggers, and 1xCTS repeats were cloned in mammalian-expression vectors by inserting annealed single-stranded DNA oligos (IDT) into appropriate plasmid backbones. iSBH sequences, as well as sgRNA spacer sequences, were cloned between BbsI restriction sites in the pcDNA3.1-U6_sgRNA_6xT-SV40_iBue_PA plasmid, generated by sequences were cloned in p035_pause-HBG-CFP-pA (XbaI, AscI restriction sites; plasmid received as a gift from Dr. David Knapp).



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